RNA

Part:BBa_K4757024

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-03)


sRNA seed region targeting BBa_J61100-mKate2

Seed region for sRNA mediated knock-down targeting 12 bp of the 3' UTR (the 5' end of BBa_J61100) and 13 bp targeting the first 13 bp from the mKate2 CDS (5'-end). The dimerization of the seed region and target RNA has a predicted binding energy of -38,93 kcal/mol, calculated with the IntaRNA tool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None